Neuronal networks in 3D hydrogel

Peter Molnar
Anupama Natarajan, Ph.D. student
Tao Xu, Ph.D. student
IDEA

 

 

Compared to the traditional 2D culture, 3D in vitro culture of neural cells more closely mimics in vivo neuronal microenvironments, and is holding huge potentials for building 3D neuronal circuits for brain research, for creating tissue-based biosensors, and for promoting systemic restoration of severe nerve injuries.

GOAL   Development of 3D engineered neuronal networks, also to stabilize 2D patterned networks
APPROACH   1. Neurons are plated in 3D collagen hydrogel

2. Patterned neurons are coated with 3D collagen hydrogel
RESULTS  

Embryonic hippocampal cells are maintaining their physiological properties in 3D hydrogels

3D                                                        2D
WORK IN PROGRESS   Using collagen hydrogels to stabilize neuronal patterns and to improve extracellular electrophysiological recordings with hydrogel coating